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Light increases the gap junctional coupling of retinal ganglion cells

机译:光增加了视网膜神经节细胞的间隙连接偶联

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摘要

We examined the effect of light adaptation on the gap junctional coupling of α-ganglion cells (α-GCs) in rabbit and mouse retinas. We assayed changes in coupling by measuring parameters of tracer coupling following injection of α-GCs with Neurobiotin and the concerted spike activity of α-GC neighbours under dark- and light-adapted conditions. We found that light adaptation using mesopic or photopic background lights resulted in a dramatic increase in the labelling intensity, number, and spatial extent of ganglion and amacrine cells coupled to OFF α-GCs when compared to levels seen under dark adaptation. While this augmentation of coupling by light did not produce an increase in the concerted spontaneous activity of OFF α-GC neighbours, it did significantly increase correlated light-evoked spiking. This was seen as an increase in the number of correlated spikes for α-GC neighbours and an extension of correlations to second-tier neighbours that was not seen under dark-adapted conditions. Pharmacological studies in the rabbit retina indicated that dopamine mediates the observed changes in coupling by differentially activating D1 and D2 receptors under different adaptation states. In this scheme, activation of dopamine D1 receptors following light exposure triggers cAMP-mediated intracellular pathways resulting in an increase in gap junctional conductance. Overall, our results indicate that as we move from night to day there is an enhanced electrical coupling between α-GCs, thereby increasing the concerted activity believed to strengthen the capacity and efficiency of information flow across the optic nerve.
机译:我们检查了光适应对兔和小鼠视网膜中α-神经节细胞(α-GCs)的间隙连接偶联的影响。我们通过测量用神经生物素注射α-GC之后的示踪剂偶联参数以及在暗光条件下的α-GC邻居的协同刺突活性来测定偶联剂的变化,从而测定了偶联的变化。我们发现,与在暗适应下观察到的水平相比,使用中视或近视背景光进行的光适应导致与OFFα-GC偶联的神经节和无长突细胞的标记强度,数量和空间范围的显着增加。虽然这种光耦合的增强并未使OFFα-GC邻域的自发一致活动增加,但确实增加了相关的光诱发的尖峰。这被认为是α-GC邻域相关尖峰数量的增加,以及在暗适应条件下未发现的与二线邻域相关性的扩展。兔视网膜的药理研究表明,多巴胺通过在不同适应状态下差异激活D1和D2受体介导观察到的偶联变化。在该方案中,曝光后多巴胺D1受体的激活触发cAMP介导的细胞内通路,从而导致间隙连接电导增加。总体而言,我们的结果表明,随着我们从黑夜到白昼的移动,α-GC之间的电耦合增强,从而增强了协同活动,从而增强了跨视神经的信息流的容量和效率。

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